CRISPR-Cas is a genetic editing tool that uses small RNA guides (gRNA) to precisely locate regions of interest on a DNA sequence and edit them. TeselaGen’s software offers several tools to design a CRISPR edition technique; ont of them is the CRISPR Knockin Design Tool.

Before launching the tool, yo need to upload a Genome, Genomic Region, and a Cas Enzyme. Also, make sure you have already created your gRNAs (remember TeselaGen’s software also automatically does this process). Once everything is ready, you can search it on the tools library and click on “Launch “Tool”.

After launching the tool, fill in the information required, selecting your desired genome, genomic region, and Cas enzyme.

Now, click on “Next”. The software will ask you to select a Homology Arms mode (it provides a brief description of your two alternatives).

Right after this step, you will be asked to upload an HDR Donor Content. This is a file with the sequence(s) that will replace your target region for the edition process.

Once this is uploaded, a window will appear indicating that the tool has completed its task.

A typical CRISPR Knockin Design should give you the following information: