The Molecular Biology Toolkit includes several libraries with different information about each item, and you have options to edit or open them to see more details.

Next, let's get to know the general details of every library included in the Toolkit and how to work with them.

The easiest way to import any type of data is to go to the corresponding library, locate the “New” icon on the upper part, and fill in the required information. However, if you prefer to import data in bulk, you can upload files in FASTA or CSV formats (remember that template files are available).

Notice that each library has its own format for files, make sure to add all the required information by following the file templates (available when clicking on a specific format on the window displayed to upload files). In addition, not all libraries allow you to directly upload data; instead, in some cases, a tool will be launched to add new data to it, since some of the libraries are a storage place for the output or results of a given tool.

The designs library includes entries you can manually create, automatically generate, or obtain using the available template designs (which allow you to select the Assembly Method).

A typical design will show you a table-like view, with SBOL icons representing DNA Parts on each column, and names of those parts on the rows below. Notice that you can add as many columns or rows as you need. To work with combinatorial designs, add more than one part on a single column; this will be taken into consideration when generating your Assembly reports.

Designs can be used to create DNA Assembly Reports or Schematics.

To create a J5 Assembly Report from a Design, click on the “Submit for Assembly” button on the upper right corner of the screen. This will start the J5 Run, you can track the status of it by checking your notifications (you will also receive an email when the report is complete). The time it takes to complete the Report will depend on the complexity of your design.

A typical report includes information about:

This library stores the results of the use of the CRISPR Knockin Design. An entry includes a repair template sequence for a given genomic region and cas enzyme, homology arms and a HDR donor content.

The library shows you the outputs of the Guide RNA Score Prediction Tool.

By clicking on an entry, you will obtain its details including the complete list of Guide RNA Scores (that will redirect you to the Guide RNA Sequences library).

This library will show the results of the Alignments product of the usage of the Sequence Alignment Tool.