The TeselaGen Community Edition provides a unified interface and a computing infrastructure for you to design DNA constructs, automate the generation of instructions for building DNA assemblies, design primers, and visualize and edit plasmids for free. Let's see what you can do in Community Edition.

To access the TeselaGen Community Edition, go to this link. There, you will see the launch screen for logging in or creating a new account using your email.

TeselaGen Community Edition is completely free. You can start by creating an account with any email of your preference. However, with a free account, you don't have access to the enterprise software level features available for Commercial Use Accounts. All the free features available on Community Edition work just the same.

Some parts of this article will redirect you to more explanatory articles of each one of our available tools!

Once you log in, the landing page should look like this:

The left side panel gives you access to the toolkits. In Community Edition, you can use the Electronic Laboratory Notebook, the Molecular Biology Toolkit, and the Registry Toolkit. Also, the Landing Page shows you your recent DNA Assembly Reports, Notebook entries, Sequences, and Designs.

🚨NOTE: The Landing Page article linked shows all the available features you can find. However, the Barcodes and Labs and Projects sections from that article are only available for commercial accounts.

TeselaGen's Electronic Laboratory Notebook helps you organizing your work on different entries and visualizing your Designs, Sequences, and other biological entities to have easy access to your experiment planning and theoretical data. Click here to get to know more about the ELN!

Your biological data is organized in libraries. They contain information on all your biological data, whether it is a reagent you have in the fridge or a construct you intend to produce with an assembly. You can create new entries or upload them in bulk. The information displayed will change depending on the type of data. A typical library looks like this:

The type of data you can store includes DNA Sequences, DNA Parts, Sequence Features, DNA Part Sets, RNA Sequences, RNA Parts, Oligos, Amino Acid Sequences, and Amino Acid Parts.
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To incorporate new data into your libraries, you can either import your entities one by one or do it in bulk. Any time you want to upload several entries together, you can build the csv file directly on the pop-up window or download the available templates to create your file to upload. Notice in the image that the accepted format files have a download icon; when clicking on them, a template file will be downloaded onto your computer.

You can create, edit, and import designs for your plasmids and vectors or use pre-existing design templates for assemblies. You can select your desired assembly strategy (Gibson, Golden Gate, MoClo) and even create libraries of combinatorial designs. A design can be created from scratch or uploaded in a csv file.

We define a design as a schematic representation of a DNA assembly process in which users specify the biological parts and the order in which they appear in the final assembled construct. A combinatorial design on TeselaGen looks like this (however, in the free version, you will be able to work only with simple designs):

In addition, you can also give directions for your experimental planning, such as indicating direct synthesis firewalls and forced assembly strategies. To learn more about designs, click here to go to the main article. Every design can be submitted for a DNA Assembly Report that breaks down your design and gives you the input pieces, oligos, and PCR reactions needed to assemble it in your lab.

This tool creates a Design from a list of Digest Parts. Parts detected as vector backbones as placed in the first column, and subsequent parts are ordered into columns based on matching 5' to 3' overhangs.

This tool takes processed gRNA sequences for a given genomic region and cas enzyme, homology arms, and HDR donor content. It will create a repair template sequence and an edited genomic region. It takes a Genomic Region, gRNA, Cas Enzyme, Homology Arms, and HDR Donor sequence as inputs to create a repair template sequence and an edited genomic region. To learn how to use this tool in detail, go to this article.

This tool proposes gRNA sequences for a given target sequence, microbial material, and a Cas enzyme. For each gRNA sequence given, it predicts the on target score, off target score, KO score, the binding site and a proposed TeselaGen ranking.

Its inputs are a microbial material, target sequence and a Cas enzyme, and gives gRNA sequences and scores as an output. Click here to learn more on the use of this tool.

This tool allows you to select sequences to order linear or double-stranded DNA fragments from IDT or Twist. The tool scores selected sequences to ensure they meet the vendor's build constraints, prices the sequences, and outputs an order form.
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