Elution Tool

Use this tool to remove, extract or separate your material of choice.

Written by Eduardo Abeliuk
Updated over a week ago

The Elution Tool accepts input column plates (attached to collection plates) and a reaction map and outputs a worklist transferring contents from the column plates to the attached collection plates.

You can access this tool in the Build platform by clicking on Tools from the top navigation bar and typing it on the Tool Library search bar as shown below.

Once you've found the tool, you can click on Launch Tool to access it.

Select the reaction map from which you want to "elute" your material of choice. We are going to consider a protein purification reaction map for this example.

Click on Select Reaction Map and select one from the list in the dialog box. Once done click Select Reaction Map in the dialog box.

In the next step you have to select the column plate for elution.

Note: The column plate should be connected to the collection plate in order for the elution step to be executed.

Creating a Collection Plate:

BUILD > Inventory > Plates > New Plate

To create a collection plate, click on Inventory from the top navigation bar in the BUILD module and select plates from it.

Click on New Plates as indicated by 3 in the image below and a dialog box will appear.

Name the plate > Select the plate type > Indicate the Lab you're affiliated with > Click on Submit, as indicated by 4, 5, 6 and 7.

Once done make sure you have selected both your column plate and collection plate and inserted the volume of the amount of elution solution to be added to each destination plate well.

You will move to the Review Worklist screen once you click the Next button and here you can check the source plate and the destination plate of your filtered/separated material.

Press the Generate Worklist to complete the tool.

You can click on the Launch Execute Worklist Tool button to directly implement your new worklist from here.

Select your worklist and here press the Next button.

If your source plates and aliquots have any extended properties, you can select or de-select the properties which you want or don't want to transfer, and then press the Next button.

In the Validate Worklist tool you can view the reaction verification information and see which materials were eluted on the collection plate and if all the reactions were validated. Once done press the Execute Worklist button.

If you want to view the updated plates with both the elution material and the source column plate, you can click on the blue "Updated 1 Plate(s)" text and view them in a new tab.

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