Contents of this Article
Workflows and Tools
TeselaGen assists you in automating processes with iterative steps to update your Lab Inventory Management System automatically with the input reagents or materials used and by adding the outputs of those reactions on existing samples, or even creating new plates or tubes with your new output materials. This is carried out through workflow definitions, where you describe the steps followed in your protocol as a series of tasks to accomplish, which we call Tools. You can find them under the Tools library in the left side panel.
Elution Tool
The Elution Tool takes plates (attached to collection plates) and a reaction map as inputs and creates a worklist transferring contents from the original plates to the attached collection plates.
After launching the tool, we first need to select the reaction map from which we will elute our material.
For this example, let's use a protein purification reaction map.
Right after that, In the next step, we will select the column plate for elution.
🚨NOTE: The column plate should be connected to the collection plate in order for the elution step to be executed.
☝️ How do I create a collection plate?
For this tool to work, we must habe a collection plate. Let's quickly create one for this example. We will open the Plates library from our Inventory.
There, we click on the "New Plate" button and fill in the required information.
Once done, we can use that plate to collect our eluted samples. On the tool, we must make sure that we selected both the column plate (1), the collection plate (2), and the volume of the amount of elution solution to be added to each destination plate well (3).
Once everything is correct, we can click on "Next" and go to the Review Worklist step.
If everything is correct, we can click on "Generate Worklist" to finish the process of the tool. A new window will indicate us that the Elution process is complete.
However, remember that the output of this tool is only a worklist that will not update our inventory unless it's executed. Let's briefly see how to execute it to actually update our plates in the inventory.
Executing the Worklist
After running the Elution Tool, we will be suggested to run the Execute Worklist Tool. However, we can also find this tool under the Tools library. When launching it, we will select the Elution Worklist we just created (if we launch the tool right after the elution step, the worklist will be automatically selected).
If the source plates and aliquots have any extended properties, we can select or de-select the properties which we want or don't want to transfer, and then press the "Next" button. The screen to validate the worklist will show the reaction verification information (which we already reviewed before).
If everything is correct, we can press the Execute Worklist button.
After the process is finished, the plates will actually be updated. We can check them by clicking on "Updated 1 Plate(s)":