This tool takes in one or more worklists with optional reaction maps to be executed in the lab. The tool validates that the source and destination materials both exist and have the appropriate volumes/capacity. If the worklist is valid, executing the tool updates the affected plates in your lab's inventory.
Input: Worklist(s), Reaction Map(s) (optional)
Output: Updated source plates and destination plates
You can find more detailed definitions of the terms mentioned in this article in the glossary.
Prerequisites:
(required) The plates to be updated must be in inventory. Go to 'Inventory > Plates' to check that both source and destination materials exist. In the screenshot below we are checking that the output plate from a bacterial transformation does indeed exist and is ready to go.
(required) The actual worklist you wish to execute.
(optional) A corresponding reaction map.
You can find the Execute Worklist tool under Tools > Tool Library. To launch the tool, click ‘Launch Tool’.
The tool will walk you through several steps to execute the worklist. You need to complete each step before proceeding to the next. After completing a step, the completed step mark (circle) will turn green with a checkmark. You can always use the ‘previous’ button to go back to any previous step.
1. Select worklist: In this example, we will be selecting the worklist named 'Microbial Transformation Worklist'. We select the worklist by checking the box next to the worklist name and then using 'Select Worklists' to continue.
2. View the selected worklist: The next screen will show us the worklists we have selected, in this case just one. Click the 'eye' icon to get a look at the worklist.
3. Link worklist to reaction map: If you wish to link to an optional reaction map, click the 'Link' button and pick the appropriate reaction map.
4. Add reagents to destination wells (optional): Note that you can add reagents to the destination wells at the time of execution of the worklist.
5. Worklist validation: Once you have worked through any possible optional features you will be presented with a validation screen where you can execute the worklist, thereby committing changes to your plates to the database. Also provided as a convenience feature is a graphical representation of the plate that can be used to track 'by hand' manual transfers according to the worklist.
6. Execute worklist: Once the worklist is complete you will be notified with a screen that allows you to visit the updated plate(s), return to the tool library or restart the tool.