Contents of this Article
Workflows and Tools
TeselaGen assists you in automating processes with iterative steps to update your Lab Inventory Management System automatically with the input reagents or materials used and by adding the outputs of those reactions on existing samples or even creating new plates or tubes with your new output materials. This is carried out through workflow definitions, where you describe the steps followed in your protocol as a series of tasks to accomplish, which we call Tools. You can find all tools under the Tool library in the left side panel.
How to use the Tool?
This tool takes in plates or tubes from your inventory and adds specific reagents to the wells with aliquots, excluding empty wells. Both plates and reagents must already be in your inventory (with enough reagent to add).
After launching the tool from the library or as a part of a workflow, you will see this screen:
Select the plates and/or tubes you want. They will appear on the list, and you can change, delete, or see them using the corresponding buttons or the eye icon.
☝️ Can I update several plates at the same time?
Yes! You can select several plates and/or tubes on a single run. Also, you can add more than one reagent.
When you're done with your selection, click on Next. This will take you to this screen:
Once you select your reagents, a new block will appear asking for specifications.
Currently, entering a working concentration will add the information to the Additives section of an aliquot but will not be used in any calculations of diluting stock solution.
☝️ What if I only want to add the reagent to specific wells?
If you don't want select all wells, but specific ones, press Ctrl/Cmd + click on the specific wells you want, or use Shift + click to select an entire row.
Remember that TeselaGen differentiates between Reagents (from the Materials Registry) and Reagent Lots (from your Inventory). A reagent indicates that you have it physically on your lab, but a reagent lot has a specific volume available. If a reagent lot is specified, the transferred volumes will be subtracted from the volume of the reagent lot. If the user chooses to simply select a reagent without specifying a reagent lot, the tool assumes that there is no limit on the reagent’s availability.
☝️ Why are my reagents not being added?
Remember that this tool takes availability information from your inventory (for reagent lots). If you intend to add more reagent than physically available, the tool will fail to add it.
☝️ How can I add different volumes of a reagent to different wells?
This tool adds the same volume of across all indicated wells. If you want to add different volumes you will need to run the tool again for each desired volume.
Click Submit to add the reagent. After this, the confirmation screen will appear.
From there, click on "Updated 1 Plate(s)" to view the changes to your plate. Double-click on any of the colored wells in the plate map or any of the rows in the table view to see details about the well contents.
From the confirmation screen, click on "Created 1 Add Reagents List" to view the list of transfers.
