A genome is a larger DNA sequence that represents the complete set of genetic material present in an organism. It contains many DNA Parts that can be identified as coding sequences, promoters, operons, etc. The large amount of information contained in a genome makes it a complex entity to work with, as there are different processes that can be carried out with them. In this article, we'll see what we can do with genomic entries on TeselaGen, which we can find under the Genomes library (part of the Registry Toolkit).

A Genomic Reference Sequence is used when creating RNA guides. By using it, the software estimates their quality as a function of potential shims outside the target area. This can be understood as the “final DNA” of a genome, made of multiple genomic regions and subject to any changes made therein.

You can view the library on the “Molecules” group on the left panel.

To create a new one, click on the green “New” icon and upload your file.

Once you’ve uploaded your genomic reference sequence, you will see it on the corresponding library.

Another file you can associate with a genome is a Genomic Region, which represents a specific part of the genome that you want to work with. Since this is only a part of a whole sequence, a single genome can have multiple genomic regions associated. To upload a genomic region, go to the “Molecules” group on the left panel and locate the Genomic Regions library.

To create a new one, click on the green “New” icon and just upload your file. Notice that this window gives you the option to associate your new genomic region with an existing genome.

Once your file is uploaded, you will see a window indicating that the new sequence was created. Here, you can see messages like the one in the image below, where TeselaGen’s platform automatically detects stop codons and notifies you to review your sequence.

A genome represents the full set of genetic material of an organism. To upload a genome, locate the “Genomes” library on the “Molecules” group on the left panel and click on the green “New” icon.

When uploading your file, you can associate your genome with an existing Genomic Region, Genomic Reference Sequence, or both. By doing this, your genome will have all the needed associations to work with the available tools. Remember, you can also edit any of them (genome, regions, or reference sequence) to change associations or edit information (for example, add new genomic regions).

Before working with tools, it is necessary to indicate to the platform that your Genome is actually a Genomic Material. To do this, go to “Genomic Materials” on the “Materials” group in the left panel.

Once you see the library, add a new material and fill in the required information. Indicate you want to use a registered genome and then select it from the list that will appear after hitting the “next” option.

Once your genomic material has been created, you will see a window with the complete information of it.

A Microbial Material is a physical entity to work with. Although a Genome is an abstract entity, if it is physically available, you can register it as a Microbial Material by going to “Microbial Materials” on the “Materials” group in the left panel.

Follow the same procedure to add a new material, selecting the existing genome you want to use. On this step, you can choose a bacterial strain and add plasmids if you eed it.

Once your material is submitted, you’re all set to start working with your genome and use the available tools.