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Example BUILD Workflow - Import Design
Example BUILD Workflow - Import Design

Building constructs from a combinatorial Golden Gate design through PCR and Assembly

Written by Tiffany Dai
Updated over a week ago

From the Design module navigate to designs and load the Combinatorial Golden Gate design.

You will be brought to the Design editor with the example design you just loaded open for editing. Since this design is ready to submit, let's do so by simply clicking the ‘Submit for Assembly’ button on the upper right.

Once submitted, the design will be sent to the assembler and after a short time, an assembly report is generated with the key information needed to do the assembly. In this case, the assembly will rely on generating DNA assembly fragments via PCR, so detailed PCR information is included in the output report. When the report is ready to view, you will see an alert. You can also check the progress of the submission via the "Tasks" monitor. To view the report, click on the appropriate row in the Tasks monitor report or the Notifications report.

To view a construct, double-click on a row in the ‘Assembled Constructs’ table. We will use this assembly report from Design to inform our workflow run in Build. The assembly report contains instructions, such as which materials are necessary for PCR reactions and assembly reactions, leading to the target constructs.

Importing a DNA assembly report to the Build module

In order to import this assembly report into the Build module, use the module switcher on the left margin to navigate to the Build module:

  • Navigate to Data > DNA Assembly Reports (j5).

  • Click on ‘Import Report From Design’.

  • Select the recently generated combinatorial Golden Gate assembly report and click 'Submit'.

  • The newly imported assembly report will now appear in 'DNA Assembly Reports (j5)' and can be used to provide necessary seed data for DNA assembly workflows.

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