The DNA Assembly Construct Selection tool allows you to select the DNA constructs you wish to build. You can choose constructs to build de novo from the output report from the j5 protocol generator. You can also check to see what you have in stock, saving you the time and expense of remaking those pieces of DNA.
Input: DNA Assembly Report
Output: DNA Assembly Construct List and Assembly Piece List
You can find the DNA Assembly Construct Selection tool under Tools > Tool Library. Click on ‘Launch Tool’ to start using the tool.
1. Selecting the report(s). The first step is confirming the DNA assembly report(s) from which you will be selecting the DNA constructs. From here you can change the reports that you have already selected. Note that you can remove a selected report by clicking the red trash icon in the report's row.
2. Selecting the assembly pieces from the reports. The next step is to select the assembly pieces contained in the previously selected reports. This can be done in two ways - via manual selection or via CSV upload.
Manual selection. Assembly pieces combine together to form the constructs in a report.
If you don't select an assembly piece that a construct requires, then that construct will not appear in the list of Buildable Constructs.
Once we hit the "Select Pieces" button... the buildable constructs will appear.
CSV upload. You can also select your Assembly Pieces via a CSV file upload where the first column specifies the "assembly piece name" and a second column specifies the "assembly piece id". You can click the "Download Template" button to get an example CSV file. The CSV upload function is accessed from the three-dot menu at the upper right in the Assembly Pieces interface.
Confirm Selection. Once you've determined your assembly pieces you can name the various output files or rely on the auto-naming templates, then click the green submit button to move on to the next step.
You'll see a summary screen with links to the various data that this tool has generated. You can click these links to check out the data that you've just created.