As we saw previously a worklist is essentially a CSV or a text file with information (commands consisting of individual lines or records with data and instructions) for the liquid handler machines. Exporting an Echo worklist from BUILD results in a .csv file following this format:
Source Plate Name, Source Well, Destination Plate Name, Destination Well, Transfer Volume.
Transfer volumes are in nL.
Wells are identified through alphanumeric coordinates.
If you use Microsoft Excel to create or edit a CSV file for import, ensure that the standard separator for lists is a comma, and the decimal symbol is a period (i. e. in Windows, under Control Panel > Clock, Language, and Region > Change date, time, or number formats > Formats > Additional Settings > List separator & Decimal symbol). Save your edited table in CSV format before closing Excel.