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Setting and Understanding Basic Assembly Methods
Setting and Understanding Basic Assembly Methods

A brief overview of assembly methods and how to set up assembly method for a design

Michael Fero PhD avatar
Written by Michael Fero PhD
Updated over a week ago

Teselagen’s design module provides the following basic assembly methods to DNA products: Mock Assembly, Golden Gate, and Gibson. 

Mock Assembly: In a mock assembly the DNA assembler outputs only the constructs that would result from your design but does not optimize for a specific assembly protocol. This is a rapid way for you to preview your constructs and refine your design without having to worry about the full experimental protocol details. 

Golden Gate (e.g. Type II Endonuclease): The Golden Gate method and its variants offer standardized, quasi-scarless, multi-part DNA assembly, and is an excellent choice for combinatorial library construction. The Golden Gate method relies upon the use of type IIs endonucleases whose recognition sites are distal from their cut sites. 

Gibson (e.g. Flanking Homology, SLIC, CPEC): This methods offer standardized, scarless, (largely) sequence-independent, multi-part DNA assembly. Since the starting materials and final products are the same for Flanking Homology, SLIC and CPEC, Teselagen provides a single design process for all of them.

By default, the assembly method is Mock Assembly. Once a design is created in the design editor (See Tutorial: A Small DNA Library Part 1 and Part 2), you can change the assembly method in the “Assembly Reaction Details”.


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