A fast and commonly used way to first verify the identity of a DNA piece for posterior work is using a restriction enzyme to create a digestion and run the samples on an electrophoresis gel.

For this example, we can select any DNA piece we want (for example, this pET28a vector in the picture below). On the Open Vector Editor view, click on Tools > Simulate Digestion to start.

A new panel called "New Digest" should open up in the Editor, where you can select your restriction enzyme and ladder. In this example, no result is shown because the selected enzymes create so many cuts, and we need to filter more.

The default selection shows Single Cutters, however, you can select from a long list of enzymes (and even add more); you can see the amount of cuts each one creates from the list. For this example, let's use AcsI.

After setting this up, you should see the expected result of your digestion like this:

If you place the cursor over a band, you will see the exact size of it and the enzyme that cut (this is useful if you are simulating a digestion with several enzymes).

If we click on it, it will select the specific region in our sequence map (from which we can create a part, primers, or any other function of the OVE):

Additionally, you can click on the "Digest Info" tab to see a table with the complete summary of your results:

The "Show Partial Digests checkbox" allows you to visualize what your digest would look like if the digest doesn't run to completion. Check how our example changes if we select this: