TeselaGen assists you in automating processes with iterative steps to update your Lab Inventory Management System automatically with the input reagents or materials used and by adding the outputs of those reactions on existing samples, or even creating new plates or tubes with your new output materials. This is carried out through workflow definitions, where you describe the steps followed in your protocol as a series of tasks to accomplish, which we call Tools. You can find them under the Tools library in the left side panel.

This tool uses the PCR Reaction Table generated on a DNA Assembly Report as input to generate plate maps with your primers and template sequence. You can manually assign the PCR reactions to locations in a PCR plate or use our algorithm to automatically assign them in a way that optimizes the PCR reactions when using a zone gradient thermocycler.

The first step is to launch it from the Tools library.

The tool consists of three steps. Now, let's see how to proceed with each one of them.

We will first select (or upload) a DNA Assembly Report.

Once selected, a list of PCR reactions associated with the selected assembly report will appear. Choose which PCR reactions you would like to run. A green checkmark indicates that the sequence exists in inventory. On this case, we will select 10 different PCR reactions and click "Next".

On step 2, we will select the plate format (here we will use a 96-well plate).

Next, we will determine the layout of our PCR reactions. By default, reactions will be distributed based on the temperature information from the selected assembly report. However, other distribution options are available, including disabling temperature zones. Once we have arranged our wells as we want, we will click "Next" to proceed to the next step.

In this step, we can select PCR materials (primary templates and primers) that are already available in the inventory. This step asks us to select aliquots for the primary templates and primers.

This step can also be automated by clicking the dropdown below the section description.

"Last Modified" will choose samples most recently updated or created, while "Fewest Plates" will choose a set of samples from the fewest number of plates. For this case, we have selected the last modified samples.

It is possible that you don't have the required materials in your inventory. This will be shown in the form of a warning, and an Order List will be generated as another output of the tool. These lists can be used in the Bioshop.

Finally, we can review the names for the output data and click "Submit."

After a few seconds, the Tool will generate all of the outputs.

This tool's output represents a plan for your PCR reactions. This helps you verify which materials are already in your inventory and which ones you need to order. This will create the plate maps but will not update anything in your inventory. To update materials in the lab (take volume from your oligo's aliquots, add new materials created after the PCR, etc.), you will need to run the Run PCR Tool.