TeselaGen assists you in automating processes with iterative steps to update your Lab Inventory Management System automatically with the input reagents or materials used and by adding the outputs of those reactions on existing samples or even creating new plates or tubes with your new output materials. This is carried out through workflow definitions, where you describe the steps followed in your protocol as a series of tasks to accomplish, which we call Tools. You can find all tools under the Tool library in the left side panel.

TeselaGen’s partition tool is a feature that allows you to divide DNA sequences into fragments. Then, your LIMS is checked to reuse the parts (if they already exist in your inventory) or create synthesis fragments. Before launching the tool, make sure your parts to be digested are already available in your libraries, and launch it:

Select your Sequences or DNA Parts and change the names if needed. Once this is done, the "Single Design" option will appear. This allows you t indicate if you want to create one design per part selected or a single one for all of them.

Once you have selected them, the next step is to indicate the partitioning parameters:

🚨NOTE: If you select type II endonuclease Assembly Strategy, the options below will change for you to indicate the specific enzyme being used.

Next, you can indicate partitioning constraints and select if you want to use aliquots already existing in your inventory.

After you have adjusted your parameters, click on “Submit” to start the process:

You can keep track of your progress in the “Tasks” section. The amount of parts and complexity of your design will also depend on the parameters you established at the beginning. With this done, you can work with it as with any other design.

If one of the parts generated is available in your inventory, PCR will be automatically selected as the assembly strategy, and it will show a small gray square on the left upper corner; on the other hand, if the option is direct synthesis, it will show a blue square on the same position. You can click on “Submit for Assembly” to run a J5 report assembly. This tool of TeselaGen’s software carries out a detailed analysis of your design to give you some insights about your assembly.

Some of the information you will receive from this analysis is: