SeqAnnotate is a powerful tool that leverages the BLAST server to automatically annotate DNA sequences, allowing you to add features or parts to target sequences with ease. You have control over the annotation references that guide the tool’s process, selecting from a curated reference database, existing annotations, or custom annotation groups to serve as the foundation for accurate annotations. SeqAnnotate also offers advanced configuration options, including partial match analysis, enabling users to find meaningful alignments even when sequence similarity is incomplete.

Input: Sequence Files

Output: Annotated Sequences (with new Features or Parts)

Access it via Tools on the left menu and find it in the Tool Library search bar.

Once you launch the SeqAnnotate tool, you'll see the steps needed to complete the annotation work.

The first step allows you to configure the target sequences (where the annotations will be created). Then, annotations could be created as Features (default) or as Parts.

If you select Features, you may select a default annotation type (which will be used when the reference sources don’t include the types).

The second step enables you to select the database of sequences. By default, the search will be performed against existent reference sequences (seeded databases at BLAST worker) and any existent FeatureGroup and PartGroup. Additionally, you may add the list of features, parts, and oligos as sources. In this case, we'll use the Reference Database.

As you can see, in "Additional Parameter," you can change the minimum identity. This is helpful for identifying partial matches.

Let's proceed with 95%.

This last step allows you to check the tool's results and manually select the annotations that won’t be created. The user may inspect all the annotations.

The first part will show you the existing annotations in the selected sequences.

Then, you'll see the new annotations found and their identity percentage.

You can find the annotated sequences by clicking on the generated link.

Your sequences will be updated in your DNA sequences library in Molecules.