This tool takes one or more nickel column plates (attached to collection plates) of microbial materials and the protein purification reaction map as input and outputs an updated collection plate of protein materials.
You can find more detailed definitions of the terms mentioned in this article in the glossary.
Input: Protein Purification Reaction Map (required), Column Plate of Microbial Materials (required)
Output: Updated Column and Collection Plates of Protein Materials
You can find the Protein Purification tool under Tools > Tool Library. Click ‘Launch Tool’ to start using the tool.
This tool consists of one step, in which the user selects a protein purification reaction map and a column plate before specifying the amount of volume to be transferred to the attached collection plate.
Step 1: Select Inputs
Begin this step by selecting a Protein Purification Reaction Map. To create a Protein Purification Reaction Map, see the documentation for the Create Reaction Map tool.
After selecting a Reaction Map, confirm that the input and output materials are correct.
Next, select a Column Plate from inventory. The Column Plate should have the extended properties "Clean" (set to true) and "Number of Uses". The Column Plate should also be attached to a Collection Plate. Finally, the Column Plate should contain the microbial materials listed in the selected Reaction Map above. For help creating and attaching a Column Plate, see the "Creating a Column Plate" section at the bottom of this page.
After selecting a Column Plate, provide a volume for the protein material to be added to the attached Collection Plate and click "Submit".
Once completed, the Column Plate should be empty, the Collection Plate should have the expected volume of protein materials.
Creating a Column Plate
To create a column plate, go to Inventory > Plates and click "New Plate" and select a column plate type. A column plate type can be created in App Settings > Plates.
A Column Plate can be attached to a Collection Plate by going to the Column Plate's page and clicking the "Attach Plate" button, then selecting any plate of the same format (Eg. Same Number of Wells.
The necessary extended properties can be added to the Column Plate from the Column Plate's page by clicking "Update" then "Update Extended Properties". From this dialog, select an extended property, provide a value and click "Add Extended Property".
