The Desalt Tool takes in one or more column plates of protein materials and outputs an updated collection plate of the same protein materials. It also allows the user to add extended properties to the collection plate.
Desalting is used to remove salts from protein solutions, phenol, or unincorporated nucleotides from nucleic acids or excess crosslinking or labeling reagents from conjugated proteins.
Input: Column Plates
Output: One or more collection plates of protein materials.
You can find more detailed definitions of the terms mentioned in this article in the glossary.
Prerequisites:
(required) The materials must exist in a materials library (Materials > DNA Materials, Microbial Materials, Cell Cultures, Protein Materials, Reagents)
If the materials do not exist yet, go to Materials > DNA Materials, Microbial Materials, Cell Cultures, Protein Materials, or Reagents > New or Upload.
You can find the Create Plate Map tool under Tools > Tool Library. Search for 'Desalt' then click on ‘Launch Tool’ to start using the tool.
The tool will walk you through several steps. You need to complete each step before proceeding to the next. After completing a step, the completed step mark (circle) will turn green with a checkmark. You can always use the ‘previous’ button to go back to any previous step.
Select Plates: The Desalt tool will only look for plates that are registered in the system as 'Column Plates'. Choose from the available column plates shown in the 'Select Plates' dialog.
Note for New Instances of the Platform:
To create a column plate, you must add the 'Clean' and 'Number of Uses' extended properties to a conventional plate. Otherwise, the plate will not show up in the Desalt 'Select Plates' dialog.
To create those extended properties, you must go to Admin User > App Settings > Extended Properties and create the two extended properties. 'Clean' will have Extended Property Class = Basic, Type = Flag, Item Type = Plate. 'Number of Uses will have Extended Property Class = Basic, Type = Number, Item Type = Plate)
The screenshot below shows what the Admin user should see if these extended properties are set up correctly.
Once you have selected plates, if they have the proper extended properties 'Clean' and 'Number of Uses', and if the plate has had an aliquot assigned to it, you will see the following:
2. Update Extended Properties: If you wish you can propagate extended properties to the output plates.
Submit: Once you submit the Desalt becomes recorded in the database and the plate contents and status are appropriately modified.
You can check the new status of the output plates, for the collection Plate as shown below:
And for the Column Plate as shown here:
