A DNA Assembly method is a technique used in laboratories to join together many DNA pieces. Different protocols can be used depending on the intended use of the final construct and other considerations like the cost, efficiency, reactants, and organisms being used. TeselaGen’s platform provides the Mock, Golden Gate, and Gibson assembly methods with the option of creating combinatorial designs. 

To see the available list of methods you have two options:
1) On your "Designs" library, you can load the example designs by clicking on the "New Design" option and selecting your preferred method.
​

2) When working with a design, click on the "Assembly Reaction Details" on the right sidebar, and select your desired method.

In a mock assembly, the DNA assembler outputs only the constructs that result from your design but does not optimize for a specific assembly protocol. This is a rapid way for you to preview your constructs and refine your design without worrying about the full experimental protocol details. This is the default method used in TeselaGen's platform for a blank design, but remember you can change it for any of the other two methods.

The Golden Gate method (e.g. Type II Endonuclease) and its variants offer standardized, quasi-scarless, multi-part DNA assembly, and are an excellent choice for combinatorial library construction. The Golden Gate method relies upon the use of type II endonucleases whose recognition sites are distal from their cut sites. 

(e.g. Flanking Homology, SLIC, CPEC): These methods offer standardized, scarless, (largely) sequence-independent, multi-part DNA assembly. Since the starting materials and final products are the same for Flanking Homology, SLIC, and CPEC, Teselagen provides a single design process for all of them.